Coronavirus information for participants
The onsite course and conference programme at EMBL has been paused until the end of June 2020.
We aim to continue offering our advanced training for the scientific community however we safely can. While some events have been cancelled, many have been rescheduled for a later date and others will be delivered as virtual events.
Registration is open for onsite courses and conferences starting after 1 July and for the virtual events. All registration fees for any events which don’t take place due to the COVID-19 disruption are fully refundable.
More information for participants of events at EMBL Heidelberg can be found here.
Course Overview
30% of the course programme covers lectures and tutorials on generating transgenic mice, and corresponding microscopy and micromanipulation techniques by experts in the field. The other 70% focuses on hands-on practical work organised in small groups. These practicals will address injection of nucleic acids into the pronucleus and cytoplasm of fertilised mouse oocytes, embryonic stem cell transfer into blastocysts, piezo-assisted stem cell transfer into 8-cell stage embryos and microscopy training.
Audience
This course is aimed at technicians and scientists interested in the generation of transgenic animals. Participants should have basic experience with eukaryotic cells and microscopy.
Modules/Resources
- DNA/RNA Microinjection into zygotes
- Contrast techniques
- Embryonic stem cell transfer
- Piezo-assisted micromanipulation
Learning Outcomes
The aim of the course is to provide participants with hands-on tutorials and the theory behind generating transgenic animals. Microinjection into mouse zygotes will be covered, as well as cell transfer into embryos and blastocysts. This course is co-organised with Eppendorf.
What Past Participants Say about the Course
"The lecturers had a huge theoretical knowledge and were able to transfer knowledge in a simple way." - Patrycja Daszczuk, University of Warsaw, Poland
