Coronavirus information for participants
The onsite course and conference programme at EMBL has been paused until the end of June 2020.
We aim to continue offering our advanced training for the scientific community however we safely can. While some events have been cancelled, many have been rescheduled for a later date and others will be delivered as virtual events.
Registration is open for onsite courses and conferences starting after 1 July and for the virtual events. All registration fees for any events which don’t take place due to the COVID-19 disruption are fully refundable.
More information for participants of events at EMBL Heidelberg can be found here.
Course Overview
A rapidly increasing number of researchers aim to purify and identify extracellular vesicles and many methodologies to isolate and analyse these vesicles are available and have been implemented. This course will cover the basics, possibilities and limitations of different purification and characterisation techniques and will address strategies to understand their role in biomedical applications.
Audience
This course is intended for PhD students and Postdoctoral researchers who are interested in learning how to work with extracellular vesicles and know more about their biomedical applications.
Modules/Resources
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Extracellular vesicles (continuous and discontinuous density gradient ultracentrifugation, ultrafiltration techniques, bead-capture and precipitation techniques, size-exclusion chromatography (SEC))
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Extracellular vesicles characterisation (Immunoblot and electron microscopy, fluorescent labeling, quantitative and qualitative analysis by flow cytometry, nanoparticle tracking analysis, in vivo imaging, proteomic and transcriptomic analysis of EV)
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Extracellular vesicles composition (lipidomics, proteomics, transcriptomics)
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Extracellular vesicles and function (molecular players for extracellular vesicle biogenesis, extracellular vesicle targeting to cells, extracellular vesicles as diagnostics and/or therapeutics in pathophysiology)
Learning Outcomes
In this course we intend to make PhD students and post-doctoral researchers familiar with good-practice-guidelines and methods for purification and identification of extracellular vesicles with practical sessions included.
What Past Participants Say About the Course:
"This course was very informative and covers all the major aspects of EV research. It addresses questions of what level of quality is required for good publications but also offers relevant approaches towards analysing data and critical thinking in the EV field. Whether you are taking your first steps in this field or more experienced, this course applies to you." - Dr. Berglind Eva Benediktsdóttir, University of Iceland, Iceland
“My research is focused on understanding the role of exosomes in the progression of glioblastoma. While working on exosome-mediated regulation of angiogenesis in glioblastoma, I found significant difficulties in getting a pure population of exosomes as it is often contaminated with other biomolecules. As a result, we were in delusion whether the data which we were getting was the effect of exosomes or other biomolecules. Fortunately, I found the EMBO Practical Course on extracellular vesicles, focusing on the isolation and purification of extracellular vesicles, which was extremely important for my research work. We were trained to use cutting-edge research tools for extracellular vesicles and learned bioinformatic analysis of biomolecules that are present in exosomes. I also got an opportunity to interact with world-leading researchers and discuss my research. ” - Abir Mondal, National Centre for Cell Science, India
