Location & dates EMBL Heidelberg, Germany 15 - 20 Mar 2020 Register Now
Deadlines Application - 7 Jan 2020
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Course Overview

Over the past decades it became evident that a RNA transcripts specific amount and localisation are important in order to understand the biological function of its (non-) coding gene. Whereby state-of-the-art in situ hybridisation (ISH) technologies are key to visualise these RNAs in a given tissue or cell. This course aims to highlight three powerful RNA visualisation techniques in histological tissue samples. During this course the participants will learn to validate the cellular localisation of candidate genes from transcriptome studies through conventional ISH and, the more recent cutting-edge, smFISH (Single Molecule Fluorescent ISH) and HCR-ISH (Hybridization Chain Reaction), based RNA ISH hybridisation methods. Additionally, the course will also cover essential tissue embedding protocols that are adapted for use in visually interrogating RNA transcripts in mammalian tissues.

Audience

This course is directed to early stage scientists (PhD and Postdocs) from the fields of RNA and molecular biology as well as various fields with an interest in these methodologies.

 

EMBL Courses and Conferences are kindly supported by our Corporate Partnership Programme

Founder partners

  • Leica logo
  • Olympus logo

Corporate partners

  • 10x Genomics_120x82
  • Becton Dickinson logo
  • Bio-Rad
  • Boehringer Ingelheim logo
  • Eppendorf logo
  • GSK logo
  • Milteniy Biotec_logo120
  • Sartorius
  • ThermoFisher logo

Associate partners

  • Merck logo
  • NEB_logo_120
  • Nikon logo
  • Promega logo
  • Roche logo
  • Sanofi logo