Location & dates EMBL Heidelberg, Germany 15 - 20 Mar 2020
Deadlines Application closed
organiser_EMBO_Course_2017

Coronavirus information for participants

The onsite course and conference programme at EMBL has been paused until the end of June 2020.


We aim to continue offering our advanced training for the scientific community however we safely can. While some events have been cancelled, many have been rescheduled for a later date and others will be delivered as virtual events.


Registration is open for onsite courses and conferences starting after 1 July and for the virtual events. All registration fees for any events which don’t take place due to the COVID-19 disruption are fully refundable.


More information for participants of events at EMBL Heidelberg can be found here.

Programme

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Day 1 - Sunday 15 March 2020
TimeSpeakerLocation
12:00 - 13:30

Arrival and check-in at the ISG Hotel

ISG Hotel

14:00

Bus departure to EMBL Heidelberg

Bus stop in front of ISG Hotel

14:30 - 15:00

Welcome and some practicalities by organizers - course outline

Flex Lab A & B

15:00 - 16:30

Ice breaking activity

Flex Lab A & B

16:30 - 17:00

Coffee break

Flex Lab A & B

17:00 - 18:00

Lecture: Histology overview and applications
Emerald Perlas - EMBL Rome, Italy

Flex Lab A & B

18:00 - 19:00

Lecture: RNA detection methods
Imre Gaspar - Institute of Molecular Biotechnology, Austria

Flex Lab A & B

19:00 Bus departure to ISG Hotel Bus stop in front of the ATC Main Entrance
19:15 - 21:00 Dinner & networking
ISG Hotel
Day 2 - Monday 16 March 2020
TimeSpeakerLocation
08:35

Bus departure to EMBL Heidelberg

Bus stop in front of ISG Hotel
(Hoffmann Bus with the EES20-02 participants)

09:00 - 09:10

Overview of the day

Flex Lab A & B

09:10 - 09:30

EMBL house notes and lab safety instructions
Yvonne Yeboah and Elisabeth Zielonka - EMBL Heidelberg, German

Flex Lab A & B

09:30 - 10:45

Interactive lecture/Demo: Specimen section preparation
(topic: conventional ISH)

Flex Lab A & B

10:45 - 11:00

Coffee break

Flex Lab A & B

11:00 - 12:00

Keynote lecture: Unveiling the Spermatogonia
Elena Vicini - Sapienza University of Rome, Italy

Flex Lab A & B

12:00 - 12:45

Interactive Lecture: Introduction and theory of conventional ISH
Emerald Perlas - EMBL Rome, Italy

Flex Lab A & B
12:45 - 13:30

Lunch break

EMBL Canteen

13:30 - 16:00

Practical in groups: Conventional ISH (section pre-treatment, Proteinase K digest, acetylation, hybridization set-up)
Sara Di Persio - Centre of Reproductive Medicine and Andrology - University Hospital Münster, Germany
Chiara Morelli - EMBL Rome, Italy
Emerald Perlas - EMBL Rome, Italy

Training Lab B

16:00 - 16:30

Coffee break

Flex Lab A & B

16:30 - 17:30

Keynote lecture: Differentially localised CDC42 mRNA isoforms
Marina Chekulaeva - Max Delbrück Center for Molecular Medicine, Germany

Flex Lab A & B

17:30 - 18:30

Keynote lecture: lncRNA Xist in epigenetic regulation
Edith Heard - EMBL Heidelberg, Germany

Flex Lab A & B

18:30 - 19:30

Dinner

EMBL Canteen

19:30 - 21:00 Group Activity: Poster Session I & Discussion Top of Helix B
21:00 Bus departure ISG Hotel Bus stop in front of the ATC Main Entrance
Day 3 - Tuesday 17 March 2020
TimeSpeakerLocation
08:35

Bus departure to EMBL Heidelberg

Bus stop in front of ISG Hotel
(Hoffmann Bus with the EES20-02 participants)

09:00 - 09:10

Overview of the day

Flex Lab A & B

09:10 - 10:00

Practical: Conventional ISH (post-hybridisation washes)
Sara Di Persio - Centre of Reproductive Medicine and Andrology - University Hospital Münster, Germany
Chiara Morelli - EMBL Rome, Italy
Emerald Perlas - EMBL Rome, Italy

Training Lab B

10:00 - 11:00

Keynote lecture: Oskar transport and localisation in Drosophila Oogenesis
Anne Ephrussi - EMBL Heidelberg, Germany

Flex Lab A & B

11:00 - 11:15

Coffee break

Flex Lab A & B

11:15 - 12:00

Practical: Conventional ISH (staining set-up)
Sara Di Persio - Centre of Reproductive Medicine and Andrology - University Hospital Münster, Germany
Chiara Morelli - EMBL Rome, Italy
Emerald Perlas - EMBL Rome, Italy

Training Lab B

12:00 - 13:00

Lunch break

EMBL Canteen

13:00 - 14:30

Interactive Lecture: Introduction to Probe design
Lucia Cassella - EMBL Heidelberg, Germany
Imre Gaspar - Institute of Molecular Biotechnology, Austria
Andre Schwarz - EMBL Heidelberg, Germany

Flex Lab A & B
14:30 - 15:00

Coffee break

Flex Lab A & B

15:00 - 16:00

Practical: Conventional ISH (washing and mounting of specimen)
Sara Di Persio - Centre of Reproductive Medicine and Andrology - University Hospital Münster, Germany
Chiara Morelli - EMBL Rome, Italy
Emerald Perlas - EMBL Rome, Italy

Training Lab B

16:00 - 18:00

Practical in goups:
- smFISH (probe labelling set-up and prepare prehypridisation buffer)
-HCR-ISH (hybridization set-up)
- Probe design (labeling)
Lucia Cassella - EMBL Heidelberg, Germany
Imre Gaspar - Institute of Molecular Biotechnology, Austria
Andre Schwarz - EMBL Heidelberg, Germany

Training Lab B

18:00 - 19:00

Dinner

EMBL Canteen

19:00 - 20:30

Discussion Round: standards and controls in the ISH field

Flex Lab A & B

20:30

Bus departure to the ISG Hotel

EMBL Bus stop
(Hoffmann Bus with the EES20-02 participants)

Day 4 - Wednesday 18 March 2020
TimeSpeakerLocation
08:35

Bus departure to EMBL Heidelberg

Bus stop in front of ISG Hotel
(Hoffmann Bus with the EES20-02 participants)

09:00 - 09:10

Overview of the day

Flex Lab A & B

09:10 - 11:00

Practical in groups: probe purification 1 round start EtOH purification (measure probes)
Lucia Cassella - EMBL Heidelberg, Germany
Imre Gaspar - Institute of Molecular Biotechnology, Austria
Andre Schwarz - EMBL Heidelberg, Germany

Training Lab B

11:00 - 12:30

Practical in groups: smFISH and HCR-ISH set up: smFISH: pre hybrididation set-up for incubation over lunch
Lucia Cassella - EMBL Heidelberg, Germany
Imre Gaspar - Institute of Molecular Biotechnology, Austria
Andre Schwarz - EMBL Heidelberg, Germany

Training Lab B

12:30 - 13:30

Lunch break

EMBL Canteen
13:30 - 15:30

Practical: conventional ISH (imaging)
Sara Di Persio - Centre of Reproductive Medicine and Andrology - University Hospital Münster, Germany
Chiara Morelli - EMBL Rome, Italy
Emerald Perlas - EMBL Rome, Italy

Training Lab B

15:30 - 16:30

Keynote Lecture: Using single molecule approaches to study the life cycle of a RNA
Jeffrey A. Chao - Friedrich Miescher Institute for Biomedical Research, Switzerland

Flex Lab A & B

16:30 - 16:45

Coffee break

16:45 - 18:30

Practical: smFISH and HCR-ISH (washes and specimen mounting)

Training Lab B

18:30 - 19:30 Dinner EMBL Canteen
19:30 - 21:00 Group Activity: Poster Session II & Discussion Top of Helix B
21:00 Bus departure to ISG Hotel Bus stop in front of the ATC Main Entrance
Day 5 - Thursday 19 March 2020
TimeSpeakerLocation
08:45

Bus departure to EMBL Heidelberg

Bus stop in front of ISG Hotel

09:00 - 09:10

Overview of the day

Flex Lab A & B

09:10 - 10:30

Practical: Imaging in groups
Lucia Cassella - EMBL Heidelberg, Germany
Imre Gaspar - Institute of Molecular Biotechnology, Austria
Andre Schwarz - EMBL Heidelberg, Germany

EMBL Main Building

10:30 - 11:00

Coffee break

Flex Lab A & B

11:00 - 12:00

Practical: Imaging in groups (continued)

EMBL Main Building

12:30 - 13:30

Lunch break

EMBL Canteen

13:30 - 14:30

Keynote Lecture: ncRNA Charme in cardiac and skeletal muscle
Monica Ballarino - Sapienza University of Rome, Italy

Flex Lab A & B
14:30 - 16:00

Practical: Imaging in groups (continued)

EMBL Main Building

16:00 -16:30

Coffee break

Flex Lab A & B

16:30 -17:30

Summary of image taking

Flex Lab A & B

17:30

Bus to ISG Hotel

Bus stop in front of the ATC Main Entrance

18:30

Bus to Neckarmünzplatz and free time until dinner

Bus stop in front of ISG Hotel

19:30

Dinner at Restaurant/Vinothek Oskar

Haspelgasse 5
69117 Heidelberg

Day 6 - Friday 20 March 2020
TimeSpeakerLocation
08:45

Bus departure to EMBL Heidelberg

Bus stop in front of ISG Hotel

09:00 - 09:10

Overview of the day

Flex Lab A & B

09:10 - 10:30

Practical: Basics ISH image analysis
Imre Gaspar - Institute of Molecular Biotechnology, Austria

Computer Training Lab

10:30 - 11:00

Coffee break

Flex Lab A & B

11:00 - 12:30

Practical: ISH image analysis (incl. standards in the field)
Imre Gaspar - Institute of Molecular Biotechnology, Austria

Computer Training Lab

12:30 -13:30

Lunch break

EMBL Canteen

13:30 - 14:30

Preparing group presentations

Flex Lab A & B
14:30 - 15:00

Coffee break

Flex Lab A & B

15:00 - 16:30

Presentation of results and discussion

Flex Lab A & B

16:30 - 17:00

Wrap-up and Farewell

Flex Lab A & B

17:15

departure to ISG Hotel, Hauptbahnhof, Crowne Plaza