Location & dates EMBL Heidelberg, Germany 15 - 20 Mar 2020
Deadlines Application closed
organiser_EMBO_Course_2017

Programme

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Day 1 - Sunday 15 March 2020
TimeSpeakerLocation
12:00 - 13:30

Arrival and check-in at the ISG Hotel

ISG Hotel

14:00

Bus departure to EMBL Heidelberg

Bus stop in front of ISG Hotel

14:30 - 15:00

Welcome and some practicalities by organizers - course outline

Flex Lab A & B

15:00 - 16:30

Ice breaking activity

Flex Lab A & B

16:30 - 17:00

Coffee break

Flex Lab A & B

17:00 - 18:00

Lecture: Histology overview and applications
Emerald Perlas - EMBL Rome, Italy

Flex Lab A & B

18:00 - 19:00

Lecture: RNA detection methods
Imre Gaspar - Institute of Molecular Biotechnology, Austria

Flex Lab A & B

19:00 Bus departure to ISG Hotel Bus stop in front of the ATC Main Entrance
19:15 - 21:00 Dinner & networking
ISG Hotel
Day 2 - Monday 16 March 2020
TimeSpeakerLocation
08:35

Bus departure to EMBL Heidelberg

Bus stop in front of ISG Hotel
(Hoffmann Bus with the EES20-02 participants)

09:00 - 09:10

Overview of the day

Flex Lab A & B

09:10 - 09:30

EMBL house notes and lab safety instructions
Yvonne Yeboah and Elisabeth Zielonka - EMBL Heidelberg, German

Flex Lab A & B

09:30 - 10:45

Interactive lecture/Demo: Specimen section preparation
(topic: conventional ISH)

Flex Lab A & B

10:45 - 11:00

Coffee break

Flex Lab A & B

11:00 - 12:00

Keynote lecture: Unveiling the Spermatogonia
Elena Vicini - Sapienza University of Rome, Italy

Flex Lab A & B

12:00 - 12:45

Interactive Lecture: Introduction and theory of conventional ISH
Emerald Perlas - EMBL Rome, Italy

Flex Lab A & B
12:45 - 13:30

Lunch break

EMBL Canteen

13:30 - 16:00

Practical in groups: Conventional ISH (section pre-treatment, Proteinase K digest, acetylation, hybridization set-up)
Sara Di Persio - Centre of Reproductive Medicine and Andrology - University Hospital Münster, Germany
Chiara Morelli - EMBL Rome, Italy
Emerald Perlas - EMBL Rome, Italy

Training Lab B

16:00 - 16:30

Coffee break

Flex Lab A & B

16:30 - 17:30

Keynote lecture: Differentially localised CDC42 mRNA isoforms
Marina Chekulaeva - Max Delbrück Center for Molecular Medicine, Germany

Flex Lab A & B

17:30 - 18:30

Keynote lecture: lncRNA Xist in epigenetic regulation
Edith Heard - EMBL Heidelberg, Germany

Flex Lab A & B

18:30 - 19:30

Dinner

EMBL Canteen

19:30 - 21:00 Group Activity: Poster Session I & Discussion Top of Helix B
21:00 Bus departure ISG Hotel Bus stop in front of the ATC Main Entrance
Day 3 - Tuesday 17 March 2020
TimeSpeakerLocation
08:35

Bus departure to EMBL Heidelberg

Bus stop in front of ISG Hotel
(Hoffmann Bus with the EES20-02 participants)

09:00 - 09:10

Overview of the day

Flex Lab A & B

09:10 - 10:00

Practical: Conventional ISH (post-hybridisation washes)
Sara Di Persio - Centre of Reproductive Medicine and Andrology - University Hospital Münster, Germany
Chiara Morelli - EMBL Rome, Italy
Emerald Perlas - EMBL Rome, Italy

Training Lab B

10:00 - 11:00

Keynote lecture: Oskar transport and localisation in Drosophila Oogenesis
Anne Ephrussi - EMBL Heidelberg, Germany

Flex Lab A & B

11:00 - 11:15

Coffee break

Flex Lab A & B

11:15 - 12:00

Practical: Conventional ISH (staining set-up)
Sara Di Persio - Centre of Reproductive Medicine and Andrology - University Hospital Münster, Germany
Chiara Morelli - EMBL Rome, Italy
Emerald Perlas - EMBL Rome, Italy

Training Lab B

12:00 - 13:00

Lunch break

EMBL Canteen

13:00 - 14:30

Interactive Lecture: Introduction to Probe design
Lucia Cassella - EMBL Heidelberg, Germany
Imre Gaspar - Institute of Molecular Biotechnology, Austria
Andre Schwarz - EMBL Heidelberg, Germany

Flex Lab A & B
14:30 - 15:00

Coffee break

Flex Lab A & B

15:00 - 16:00

Practical: Conventional ISH (washing and mounting of specimen)
Sara Di Persio - Centre of Reproductive Medicine and Andrology - University Hospital Münster, Germany
Chiara Morelli - EMBL Rome, Italy
Emerald Perlas - EMBL Rome, Italy

Training Lab B

16:00 - 18:00

Practical in goups:
- smFISH (probe labelling set-up and prepare prehypridisation buffer)
-HCR-ISH (hybridization set-up)
- Probe design (labeling)
Lucia Cassella - EMBL Heidelberg, Germany
Imre Gaspar - Institute of Molecular Biotechnology, Austria
Andre Schwarz - EMBL Heidelberg, Germany

Training Lab B

18:00 - 19:00

Dinner

EMBL Canteen

19:00 - 20:30

Discussion Round: standards and controls in the ISH field

Flex Lab A & B

20:30

Bus departure to the ISG Hotel

EMBL Bus stop
(Hoffmann Bus with the EES20-02 participants)

Day 4 - Wednesday 18 March 2020
TimeSpeakerLocation
08:35

Bus departure to EMBL Heidelberg

Bus stop in front of ISG Hotel
(Hoffmann Bus with the EES20-02 participants)

09:00 - 09:10

Overview of the day

Flex Lab A & B

09:10 - 11:00

Practical in groups: probe purification 1 round start EtOH purification (measure probes)
Lucia Cassella - EMBL Heidelberg, Germany
Imre Gaspar - Institute of Molecular Biotechnology, Austria
Andre Schwarz - EMBL Heidelberg, Germany

Training Lab B

11:00 - 12:30

Practical in groups: smFISH and HCR-ISH set up: smFISH: pre hybrididation set-up for incubation over lunch
Lucia Cassella - EMBL Heidelberg, Germany
Imre Gaspar - Institute of Molecular Biotechnology, Austria
Andre Schwarz - EMBL Heidelberg, Germany

Training Lab B

12:30 - 13:30

Lunch break

EMBL Canteen
13:30 - 15:30

Practical: conventional ISH (imaging)
Sara Di Persio - Centre of Reproductive Medicine and Andrology - University Hospital Münster, Germany
Chiara Morelli - EMBL Rome, Italy
Emerald Perlas - EMBL Rome, Italy

Training Lab B

15:30 - 16:30

Keynote Lecture: Using single molecule approaches to study the life cycle of a RNA
Jeffrey A. Chao - Friedrich Miescher Institute for Biomedical Research, Switzerland

Flex Lab A & B

16:30 - 16:45

Coffee break

16:45 - 18:30

Practical: smFISH and HCR-ISH (washes and specimen mounting)

Training Lab B

18:30 - 19:30 Dinner EMBL Canteen
19:30 - 21:00 Group Activity: Poster Session II & Discussion Top of Helix B
21:00 Bus departure to ISG Hotel Bus stop in front of the ATC Main Entrance
Day 5 - Thursday 19 March 2020
TimeSpeakerLocation
08:45

Bus departure to EMBL Heidelberg

Bus stop in front of ISG Hotel

09:00 - 09:10

Overview of the day

Flex Lab A & B

09:10 - 10:30

Practical: Imaging in groups
Lucia Cassella - EMBL Heidelberg, Germany
Imre Gaspar - Institute of Molecular Biotechnology, Austria
Andre Schwarz - EMBL Heidelberg, Germany

EMBL Main Building

10:30 - 11:00

Coffee break

Flex Lab A & B

11:00 - 12:00

Practical: Imaging in groups (continued)

EMBL Main Building

12:30 - 13:30

Lunch break

EMBL Canteen

13:30 - 14:30

Keynote Lecture: ncRNA Charme in cardiac and skeletal muscle
Monica Ballarino - Sapienza University of Rome, Italy

Flex Lab A & B
14:30 - 16:00

Practical: Imaging in groups (continued)

EMBL Main Building

16:00 -16:30

Coffee break

Flex Lab A & B

16:30 -17:30

Summary of image taking

Flex Lab A & B

17:30

Bus to ISG Hotel

Bus stop in front of the ATC Main Entrance

18:30

Bus to Neckarmünzplatz and free time until dinner

Bus stop in front of ISG Hotel

19:30

Dinner at Restaurant/Vinothek Oskar

Haspelgasse 5
69117 Heidelberg

Day 6 - Friday 20 March 2020
TimeSpeakerLocation
08:45

Bus departure to EMBL Heidelberg

Bus stop in front of ISG Hotel

09:00 - 09:10

Overview of the day

Flex Lab A & B

09:10 - 10:30

Practical: Basics ISH image analysis
Imre Gaspar - Institute of Molecular Biotechnology, Austria

Training Lab B

10:30 - 11:00

Coffee break

Flex Lab A & B

11:00 - 12:30

Practical: ISH image analysis (incl. standards in the field)
Imre Gaspar - Institute of Molecular Biotechnology, Austria

Rooftop Lounge

12:30 -13:30

Lunch break

EMBL Canteen

13:30 - 14:30

Preparing group presentations

Flex Lab A & B
15:00 - 15:30

Coffee break

Flex Lab A & B

15:30 - 16:30

Presentation of results and discussion

Flex Lab A & B

16:30 - 17:00

Wrap-up and Farewell

Flex Lab A & B

17:15

departure to ISG Hotel, Hauptbahnhof, Crowne Plaza