Programme
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Please note the below programme still subject to changes
| Time | Speaker | Location |
|---|---|---|
| 08:45 - 09:00 |
Bus from ISG Hotel to EMBL ATC entrance |
ISG Hotel Bus Stop |
| 09:00 - 09:15 |
Welcome and EMBL Overview |
Coutryard Room A & B |
| 09:15 - 09:30 |
Introduction to Training Lab and House notes |
Coutryard Room A & B |
| 09:30 - 09:45 |
Welcome and Introductions |
Coutryard Room A & B |
| 09:45 - 10:45 |
Flash talks participants |
Coutryard Room A & B |
| 10:45 - 11:00 |
Coffee Break |
Coutryard Room A & B |
| 11:00 - 12:00 |
Lecture: Metagenomics: Concepts, historical milestones and next advance |
Coutryard Room A & B |
| 12:00 - 13:00 |
Lunch break |
EMBL Canteen |
| 13:00 - 14:00 |
Lecture: Metagenomics library preparation for NGS |
Coutryard Room A & B |
| 14:00 - 15:00 |
Group Activity |
Coutryard Room A & B |
| 15:00 - 16:00 |
Lecture: The impact of Metagenomics, on genes and genomes discovery |
Coutryard Room A & B |
| 16:00 - 16:30 |
Coffee break |
Coutryard Room A & B |
| 16:30 - 17:30 |
Lecture: What bring metagenomic approach in cardiometabolic and nutrition-related disorders? |
Coutryard Room A & B |
| 17:30 - 19:30 |
Poster Session |
Coutryard Room A & B |
| 19:30 - 20:30 |
Dinner |
ATC Rooftop Lounge |
| 20:30 |
Bus to ISG Hotel |
ATC Bus Stop |
| Time | Speaker | Location |
|---|---|---|
| 08:45 - 09:00 |
Bus from ISG Hotel to EMBL ATC entrance |
ISG Hotel Bus Stop |
| 09:00 - 09:15 |
Quick overview of the day |
Courtyard Room A+B |
| 09:15 - 10:30 |
Practical: Extraction of metagenomic DNA in groups from soil samples |
Training Lab A+B |
| 10:30 - 11:00 |
Coffee Break |
Courtyard Room A+B |
| 11:00 - 12:30 |
Practical: Quality control of DNA samples and Nanopore 16s PCR set up |
Training Lab A+B |
| 12:30 - 13:30 |
Lunch break |
EMBL Canteen |
| 13:30 - 14:30 |
Practical: Discussion of Extraction Results |
Training Lab A+B |
| 14:30 - 15:30 |
Lecture: Metagenomics and other culture-independent tools to study virus communities |
Courtyard Room A+B |
| 15:30 - 16:45 |
Practical: Nanopore: 16S PCR cleanup, finishing library preparation and starting sequencing run, followed by Genomics Core Facility Tour |
Training Lab A+B | Gene Core Facility |
|
Flexible Coffee Break |
Courtyard Room A+B |
|
| 17:00 - 19:00 |
Practical: Nanopore: 16S analysis/ DNA cleanup and Sequencing start |
Training Lab A+B |
| 19:00 | Dinner | EMBL Canteen |
| 19:00 | Bus to ISG Hotel | ATC Bus Stop |
| Time | Speaker | Location |
|---|---|---|
| 08:45 - 09:00 |
Bus from ISG Hotel to EMBL ATC entrance |
ISG Hotel Bus Stop |
| 09:00 - 09:15 |
Quick overview of the day |
Courtyard Room A+B |
| 09:15 - 09:45 | Practical: Test digestion conditions for metagenomic DNA and preparation of agarose gels José Eduardo González Pastor - Center of Astrobiology (CSIC/INTA), Spain Arantxa Lopez - FISABIO, Spain |
Training Lab A+B |
| 09:45 - 11:00 |
Practical introduction: Single cells genomics |
Courtyard Room A+B |
| 11:00 - 11:15 |
Coffee Break |
Courtyard Room A+B |
| 11:15 - 12:30 |
Practical: Digestion of metagenomic DNA for cloning in the libraries |
Training Lab A+B |
| 12:30 - 13:30 | Lunch break |
EMBL Canteen |
| 13:30 - 14:30 |
Practical: Purification from gel of the digested metagenomic DNA |
Training Lab A+B |
| Flexible Coffee break |
Courtyard Room A+B |
|
| 15:00 - 17:00 |
Practical: Cloning of the metagenomic DNA + overnight ligatio |
Training Lab A+B |
| 17:00 - 19:30 |
Practical (single cell microbial genomics): Droplet generation for single cell genomics and PCR set-up |
Computer Training Lab |
| 19:30 - 20:30 |
Dinner |
ISG Hotel |
| 20:30 |
Bus to ISG Hotel |
ATC Bus Stop |
| Time | Speaker | Location |
|---|---|---|
| 08:45 - 09:00 |
Bus from ISG Hotel to EMBL ATC entrance |
ISG Hotel Bus Stop |
| 09:00 - 09:15 |
Quick overview of the day |
Courtyard Room A+B |
| 09:15 - 11:00 |
Practical: Transformation in E. coli of the library |
Training Lab A+B |
| 11:00 - 12:00 |
Methodological lecture and discussion session: Metagenomics experimental design: Common shortcoming and pitfalls |
Courtyard Room A+B |
| 12:00 - 13:00 |
Lunch break |
EMBL Canteen |
| 13:00 - 14:00 |
Lecture: Hands-on/Introduction to Linux I |
Courtyard Room A+B |
| 14:00 -14:30 | Coffee break |
Courtyard Room A+B |
| 14:30 - 17:00 |
Hands-on/Introduction to Linux II |
Computer Training Lab |
| 17:00 |
Free evening with tour of Heidelberg |
|
| 17:00 - 17:30 |
Bus to ISG Hotel |
ATC Bus Stop |
| 17:30 - 18:00 |
Bus to Bismarkplatz |
ISG Hotel bus stop |
| Time | Speaker | Location |
|---|---|---|
| 08:45 - 09:00 |
Bus from ISG Hotel to EMBL ATC entrance |
ISG Hotel Bus Stop |
| 09:00 - 09:15 | Quick overview of the day Giuseppe D'Auria - FISABIO Sequencing and Bioinformatics Service, Spain |
Computer Training Lab |
| 09:15 - 10:00 |
Practical: Analysis of some colonies from the metagenomic libraries -Interpretation of results |
Training Lab A+B |
| 10:00 - 10:30 |
Coffee break |
Computer Training Lab |
| 10:30 - 12:30 | Lecture: Diversity matters, a place for a classification of uncultured microorganisms in Taxonomy Kostas Konstantinidis - Georgia Institute of Technology, USA |
Computer Training Lab |
| 12:30 - 13:30 |
Lunch |
EMBL Canteen |
| 13:30 - 15:30 |
Lecture and Hands-on: the MiGA web-server: the genome-equivalent of RDP/Silva SSU web-servers |
Computer Training Lab |
| 15:30 - 18:30 |
Practical (single cell microbial genomics): Visualization of droplets and single cell analysis |
|
|
Flexible Coffee break |
Computer Training Lab |
|
| 18:30 - 19:30 |
Lecture and Hands-on: Tools for specialized analyses of metagenomes |
Computer Training Lab |
| 18:15 - 18:30 |
Bus to ISG Hotel |
ATC Bus Stop |
| 18:45 |
Dinner & drinks/ BBQ |
ISG Hotel |
| Time | Speaker | Location |
|---|---|---|
| 08:45 - 09:00 |
Bus from ISG Hotel to EMBL ATC entrance |
ISG Hotel Bus Stop |
| 09:00 - 09:15 |
Quick overview of the day |
Computer Training Lab |
| 09:15 - 11:00 |
Lecture: Some theory on taxonomy, phylogeny and diversity measures based on 16S rRNA gene amplicons and direct metagenomic |
Computer Training Lab |
| 11:00 - 11:15 | Coffee break |
Computer Training Lab |
| 11:15 – 12:15 |
Lecture: Practical session 16S rRNA gene amplicons and direct metagenomic |
Computer Training Lab |
| 12:15 - 13:15 |
Lunch break |
EMBL Canteen |
| 13:15 – 14:15 |
Hands-on: Practical session 16S rRNA gene amplicons and direct metagenomic |
Computer Training Lab |
| 14:15 – 15:00 |
Hands-on: Introduction to 16S rDNA profiling using QIIME2 |
Computer Training Lab |
| 15:00 - 15:30 |
Coffee break |
Computer Training Lab |
| 15:30 - 18:15 |
Hands-on: Introduction to 16S rDNA profiling using QIIME2 |
Computer Training Lab |
| 18:15 - 19:15 |
Dinner |
Computer Training Lab |
| 19:15 - 21:15 |
Poster Session |
|
| 21:15 |
Bus to ISG Hotel |
ATC Bus Stop |
| Time | Speaker | Location |
|---|---|---|
| 08:45 - 09:00 |
Bus from ISG Hotel to EMBL ATC entrance |
ISG Hotel Bus Stop |
| 09:00 - 09:15 |
Quick overview of the day |
Computer Training Lab |
| 09:15 – 11:15 |
Lecture and Hands-on: Upstream analysis: data organisation and standards in metagenomics and Metagenomic assembly, ORF finding, functional annotation |
Computer Training Lab |
| 11:15 - 11:30 |
Coffee break |
Computer Training Lab |
| 11:30 - 13:00 | Practical: MiniIon data anaysis: QC and Taxonomy profiling Jan Provaznik - EMBL Heidelberg, Germany |
Computer Training Lab |
| 13:00 - 14:00 |
Lunch break |
Computer Training Lab |
| 14:00 – 15:00 |
Lecture: Analysis Tools Overview: INCA, MILC and MELP |
Computer Training Lab |
| 15:00 - 15:30 |
Coffee break |
Computer Training Lab |
| 15:30 - 17:30 |
Hands-on: INCA |
Microscopy room and Computer Training Lab |
| 17:30 -18:30 |
Presentation preparation |
Computer Training Lab |
| 19:00 - 20:00 | Dinner | |
| 20:00 |
Bus to ISG Hotel |
ATC Bus Stop |
| Time | Speaker | Location |
|---|---|---|
| 08:45 - 09:00 |
Bus from ISG Hotel to EMBL ATC entrance |
ISG Hotel Bus Stop |
| 09:00 - 09:15 |
Quick overview of the day |
Computer Training Lab |
| 09:15 - 10:30 |
Lecture and Hands-on: Functional and taxonomic analysis |
Computer Training Lab |
| 10:30- 10:45 | Coffee break |
Computer Training Lab |
| 10:45 - 12:00 | Practical: Hands-on/MEGAN Daniel Huson - University of Tübingen, Germany |
Computer Training Lab |
| 12:00 - 13:00 |
Lunch break |
EMBL Canteen |
| 13:00 - 14:00 |
Practical: Hands-on/MEGAN |
Computer Training Lab |
| 14:00 - 16:30 |
Group activity and Presentations |
Computer Training Lab |
| 16:30 - 16:45 |
Coffee break |
Computer Training Lab |
| 16:45 - 17:45 |
Wrap-up and conclusions |
Computer Training Lab |
| 17:45 - 18:15 |
Bus to ISG Hotel |
ATC Bus Stop |
| 18:15 - 18:45 |
Bus to Neckarmünzplatz |
ISG Hotel Bus Stop |
| 19:00 |
Dinner Downtown |