Location & dates Virtual 28 - 30 Oct 2020
Deadlines Registration closed Abstract submission closed
organiser_EMBO_Workshop_2017

EMBL Courses and Conferences during the Coronavirus pandemic

With the onsite programme paused, many of our events are now being offered in virtual formats.


Registration is open as usual for many events, with back-up plans in place to move further courses and conferences online as necessary. Registration fees for any events affected by the COVID-19 disruption are fully refundable.


More information for participants of events at EMBL Heidelberg can be found here.

Industry Webinar

The industry webinars will be hosted by the sponsoring company prior to the virtual EMBO WorkshopNeuroepigenetics: From Cells to Behaviour and Disease. Participation in this webinar is free of charge for registered conference attendees but the number of available places is limited (first come, first served). All conferens participants should have received an email with a registration link.

Industry Workshop by Dovetail Genomics

 

Life After ChIP-seq: Genome conformation enhances our view of the regulatory landscape

 

27 October from 16:00 to 17:00 (CET, e.g. Berlin)
To find out the equivalent time zone in your location, enter Berlin, the CET programme time and your city into the Time Zone Converter


Speaker: Cory Padilla, Ph.D. Head of Scientific Affairs, Dovetail Genomics

 

Chromatin Immunoprecipitation, coupled with sequencing, has shaped our understanding of how transcriptional machinery interacts with genomes to facilitate gene regulation. These protein-DNA interactions are fundamental components of primary-feature epigenetic signatures that are informative across development, gene regulation, and disease research. A challenge associated with these data is that they depict protein anchors in linear space and do not consider the complex and hierarchical genomic folding associated with DNA packaged in chromatin and further compacted into a nucleus. Here we combine chromatin immunoprecipitation with Hi-C in a single assay, known as HiChIP, to describe the interactome associated with protein anchors. By comparing the interactome of H3K27ac (enhancers), promoters (H3K4me3), and topological boundaries (CTCF) in iPSC and NSC, we can describe the different transcriptional landscape of the essential genes involved with pluripotency maintenance, NANOG and OCT4. We then investigate the enhancer interactome of NSC with regards to Alzheimer's polymorphisms identified through GWAS. We show four distinct enhancer regions are associated with APOE, highly implicated in Alzheimer's disease, implying that SNPs occurring kilobases away from a gene promotor could impact gene regulation.  These data introduce an additional dimension to ChIP-seq experiments to more completely describe the mechanism of gene activation and provide a more robust annotation of both protein anchors and their target gene as well as disease-associated polymorphisms.