Location & dates EMBL Heidelberg, Germany 31 Aug - 4 Sep 2020 Register Now
Deadlines Application - 8 Jun 2020
organiser_10XGenomics

Programme

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Day 1 - Monday 31 August 2020
TimeSpeakerLocation

Introductions

10:30 - 11:00

Arrival and registration

Flex Lab A & B

11:00 - 11:30

Welcome and Lab safety instructions
Katharina Danielski - 10x Genomics, Germany
Elisabeth Zielonka - EMBL Heidelberg, Germany

Flex Lab A & B

11:30 - 13:00

Introduction Visium Workflow
FAS from 10x Genomics, Germany

Flex Lab A & B

13:00 - 14:00

Lunch break

EMBL Canteen

14:00 - 15:00

Introduction to Visium Sample Preparation
FAS from 10x Genomics, Germany

Flex Lab A & B

15:00 - 16:00

Practical: Visium Accessory Kit
FAS from 10x Genomics, Germany

Training Lab B
16:00 - 17:00

Practical: Cryosectioning Demo

Training Lab B

17:00 - 18:00

Invited speaker talk

Flex Lab A & B

18:00 - 19:00

Dinner

EMBL Canteen

Day 2 - Tuesday 1 September 2020
TimeSpeakerLocation

Tissue Optimization

09:00 - 09:30

Practical: Setup Lab
FAS from 10x Genomics, Germany

Training Lab B

09:30 - 10:30

Practical: Methanol Fixation and H&E Staining
FAS from 10x Genomics, Germany

Training Lab B

10:30 - 11:30

Practical: Brightfield Imaging

Training Lab B

11:30 - 13:00

Lunch break

EMBL Canteen

13:00 - 14:00

Practical: Permeabilization Timecourse
FAS from 10x Genomics, Germany

Training Lab B

14:00 - 15:00

Practical: Fluorescent cDNA Synthesis
FAS from 10x Genomics, Germany

Training Lab B
15:00 - 16:30

Practical: Tissue removal (1 hour incubation)
FAS from 10x Genomics, Germany

Training Lab B

During incubation: Troubleshooting Slides
FAS from 10x Genomics, Germany

Flex Lab A & B

16:30 - 17:30

Practical: Fluorescent cDNA Imaging

Training Lab B

17:30 - 18:30

Dinner

EMBL Canteen

Day 3 - Wednesday 2 September 2020
TimeSpeakerLocation

Library Preparation until cDNA

09:00 - 09:30

Practical: Setup Lab
FAS from 10x Genomics, Germany

Training Lab B

09:30 - 10:30

Practical: Methanol Fixation and H&E Staining
FAS from 10x Genomics, Germany

Training Lab B

10:30 - 11:30

Practical: Brightfield Imaging

Training Lab B

11:30 - 13:00

Lunch break

EMBL Canteen

13:00 - 14:00

Practical: Permeabilization, RT and Second Strand Synthesis
FAS from 10x Genomics, Germany

Training Lab B

14:00 - 15:00

Practical: cDNA Denaturation and qPCR
FAS from 10x Genomics, Germany

Training Lab B
15:00 - 16:00

Practical: cDNA Amplification and QC
FAS from 10x Genomics, Germany

Training Lab B

from 16:00

Free afternoon

17:00

Optional: Guided tour of Heidelberg

Neckarmünzplatz
Day 4 - Thursday 3 September 2020
TimeSpeakerLocation

Finish Library Preparation

09:00 - 12:00

Library Preparation (Fragmentation, Ligation, Sample Index PCR)
FAS from 10x Genomics, Germany

Training Lab B

12:00 - 13:00

Lunch break

EMBL Canteen

13:00 - 15:00

Sequencing Facility Tour

EMBL GeneCore Facility

15:00 - 16:00

Invited speaker talk

Flex Lab A & B
16:00 - 17:00

Invited speaker talk

Flex Lab A & B

from 19:00

Course Dinner

Restaurant in downtown Heidelberg

Day 5 - Friday 4 September 2020
TimeSpeakerLocation
Data Analysis
09:00 - 11:00

Space ranger and Loupe
FAS from 10x Genomics, Germany

Flex Lab A & B

11:00 - 12:00

Invited speaker talk

Flex Lab A & B

12:00 - 13:00

Lunch break

EMBL Canteen

13:00 - 14:00

Q&A and Wrap up

Flex Lab A & B