Location & dates EMBL Heidelberg, Germany 17 - 21 May 2021
Deadlines Application closed

EMBL Courses and Conferences during the Coronavirus pandemic

With the onsite programme paused, many of our events are now being offered in virtual formats.


Registration is open as usual for many events, with back-up plans in place to move further courses and conferences online as necessary. Registration fees for any events affected by the COVID-19 disruption are fully refundable.


More information for participants of events at EMBL Heidelberg can be found here.

Registration is not yet open for this event. If you are interested in receiving more information please register your interest. 

Course Overview

Flow Cytometry is one of the most powerful single cell analysis methods used throughout the life sciences and medicine. Utilising Flow Cytometry we can identify and quantify many different parameters from heterogeneous samples, analyse the corresponding state of proliferation or simply detect the expression of fluorescent reporters. In line with the suggestions from our course in 2017, we will significantly enhance the number of analysis sessions together with our partners from FlowJo. We will cover the theory of Flow Cytometry and corroborate this information intensively with the practical issues of the most frequent assays as part of our practical course curriculum.

Audience

The course is designed for researchers at any step of their career and will cover the subject from basic principles to complex assays. Prior basic working knowledge of Flow Cytometry, data acquisition or analysis software (DIVA + FlowJo)  is of advantage, but not strictly a must.

Modules/Resources

  • In-depth principles of Flow Cytometry
  • Quality assessment and instrument validation
  • Analysis of cell cycle profiles in fixed and live cells
  • Proliferation and cell division assessment
  • Apoptosis assays
  • Tissue staining of cell surface and intracellular antigens
  • Principles and guidelines for experimental design, especially for multi-colour Flow Cytometry
  • Essential of gating and gating controls
  • Detailed multi-sessions on analysing data from Flow Cytometers in cooperation with FlowJo
  • Guidelines to prepare flow cytometry data for publication

Learning Outcomes

Our goal for this course is to teach the basic of this technology with a very big emphasis on practical work at the instruments. Nothing is better than learning by doing. We have a great set of experienced trainers in this technology delivering lectures and accompanying you into the lab doing flow on your own. We will have support from FlowJo delivering the basics and some advanced features in multiple sessions. This in-depth five-day course will push your knowledge and skills preparing you for any flow projects ahead on your research path.

What past participants say about this course

"A comprehensive and interactive course that I would recommend to everyone that uses flow cytometry in their work." - Oana Baldasici, The Oncology Institute Prof. Dr.I. Chiricuta, Romania

 

EMBL Courses and Conferences are kindly supported by our Corporate Partnership Programme

Founder partners

  • Leica logo
  • Olympus logo

Corporate partners

  • 10x Genomics_120x82
  • Becton Dickinson logo
  • Bio-Rad logo
  • Boehringer Ingelheim logo
  • Eppendorf logo
  • GSK logo
  • Milteniy Biotec logo
  • NetApp logo
  • Stilla logo
  • ThermoFisher logo

Associate partners

  • Merck logo
  • New England Biolabs logo
  • Nikon logo
  • Promega logo
  • Roche logo
  • Sanofi logo
  • Sartorius logo